Exploring detergent insolubility in bovine hippocampal membranes: a critical assessment of the requirement for cholesterol
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چکیده
منابع مشابه
Exploring detergent insolubility in bovine hippocampal membranes: a critical assessment of the requirement for cholesterol.
The phenomenon of detergent insolubility of bovine hippocampal membranes in Triton X-100 was monitored by estimating the presence of phospholipids in the insoluble pellet. This represents a convenient and unambiguous assay and reports the dependence of the extent of phospholipid solubilization on detergent concentration. The advantage of this approach is its ability to accurately determine the ...
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15 صفحه اولDetergent Insolubility and Lipid Rafts
phase domains, leaving the lo-phase domains relatively unchanged as DRMs. Both of these approaches showed that TX100 did not substantially change the relative amounts of membrane in the two phases. By contrast, using calorimetry, Heerklotz and colleagues reported that TX100 can induce phase separation in previously uniform mixtures (45, 46). However, results of the other two studies described a...
متن کاملDetergent Insolubility and Lipid Rafts
phase domains, leaving the lo-phase domains relatively unchanged as DRMs. Both of these approaches showed that TX100 did not substantially change the relative amounts of membrane in the two phases. By contrast, using calorimetry, Heerklotz and colleagues reported that TX100 can induce phase separation in previously uniform mixtures (45, 46). However, results of the other two studies described a...
متن کاملDetergent Insolubility and Lipid Rafts
phase domains, leaving the lo-phase domains relatively unchanged as DRMs. Both of these approaches showed that TX100 did not substantially change the relative amounts of membrane in the two phases. By contrast, using calorimetry, Heerklotz and colleagues reported that TX100 can induce phase separation in previously uniform mixtures (45, 46). However, results of the other two studies described a...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 2004
ISSN: 0005-2736
DOI: 10.1016/j.bbamem.2003.11.013